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枸杞属植物(Lycium haminifoliumMill)花粉粒发育成胚和植株的形成已有报导。本文报导枸杞叶外植体的愈伤组织诱导及其植株的再生。从未开花植株上摘取无病虫害的叶片作材料,先用流水冲洗除去尘土,然后在70％的酒精中浸20秒左右,再在含万分之二昇汞和5％安替福民的溶液中消毒4～5分钟(消毒时间不能过长,以免伤害叶片)。用无菌水淋洗3～5次后,在无菌条件下将叶片横切成1厘米左右的切段,接种在Murashige和Skoog培养基上,培养基附加激动素(K)2.0,吲哚乙酸(IAA)2.0或K4.0,IAA
Lycium haminifolium Mill pollen grains develop into embryos and the formation of plants has been reported. This paper reports the callus induction of Lycium barbarum explants and their plant regeneration. Insects and pests have never been blossoming from the leaves of the plant material, first rinse with water to remove dust, and then soaked in 70% alcohol for about 20 seconds, then with two million mercuric chloride and 5% Disinfection in 4 to 5 minutes (disinfection time can not be too long, so as not to hurt the leaves). After rinsed with sterile water for 3 to 5 times, the leaves were cross-sectioned into 1 cm sections under aseptic conditions and inoculated on Murashige and Skoog medium supplemented with kinetin (K) 2.0, indole Acetic acid (IAA) 2.0 or K4.0, IAA