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以设施栽培的枥乙女草莓为试材,研究蔗糖代谢和己糖代谢关键酶在草莓果实糖积累进程中的作用。采集花后1～8周的果实,分别测定果实中葡萄糖、果糖、蔗糖的含量和蔗糖磷酸合酶(SPS)、蔗糖合酶(SS)、可溶性酸性转化酶(AI)、中性转化酶(NI)、己糖激酶(HXK)及果糖激酶(FRK)等蔗糖和己糖代谢酶的活性。结果表明,草莓果实的总糖含量随果实发育持续积累,但蔗糖的快速积累对果实后期糖的积累贡献最大。在果实发育前期,草莓AI活性随果实发育而下降,但在果实发育后期,AI活力随果实成熟而急剧上升,这使果实最终以积累己糖为主。果实蔗糖快速积累期,合成蔗糖的酶SPS和SS(合成方向)的活性下降到较低水平,表明这2种酶对草莓后期果实蔗糖的快速积累贡献较少。果实发育后期较低的HXK和FRK活力有利于果实糖的快速积累。
The cultivation of stable female Teddy Bears strawberries were used as materials to study the role of sucrose metabolism and key enzymes of hexose metabolism in the accumulation of sugar in strawberry fruit. The contents of glucose, fructose and sucrose, the content of sucrose phosphate synthase (SPS), sucrose synthase (SS), soluble acid invertase (AI), neutral invertase NI), hexokinase (HXK) and fructokinase (FRK) and other sucrose and hexose metabolizing enzymes. The results showed that the total sugar content of strawberry fruits continued to accumulate with fruit development, but the rapid accumulation of sucrose contributed most to the accumulation of sugars in the late fruit. In the early stage of fruit development, the AI activity of strawberry decreased with the development of the fruit, but in the later fruit development, the AI activity rose sharply with the maturity of the fruit, which eventually led to the accumulation of hexose in the fruit. The activity of sucrose synthase SPS and SS (synthesis direction) of synthetic sucrose decreased rapidly during the rapid accumulation of sucrose, indicating that these two enzymes contributed less to the rapid accumulation of sucrose in late strawberry fruit. The lower HXK and FRK activities in the late fruit development stage favor the rapid accumulation of fruit sugars.