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以梭曼水解反应过渡态类似物 O1-甲基 - O2 -(1 ,2 ,2 -三甲基丙基 ) - 2 -羟基 - 5-硝基苯基甲基膦酸为半抗原得到了梭曼单链抗体酶 EP6.对这一株抗体进行了核苷酸序列的测定 ,测序结果表明这一单链抗体基因全长 71 4bp,其中重链长 351 bp,编码1 1 7个氨基酸 ,轻链长 31 8bp,编码 1 0 6个氨基酸 ,二者由编码 (Gly4 Ser) 3的 45bp的连接肽基因相连 .重 ,轻链均为开放读框 ,有明确的四个框架区和三个互补决定区 (CDR)以及抗体特征性的两个半胱氨酸残基 ,表明重 ,轻链的氨基酸序列符合鼠抗体可变区特征 .在 SGI工作站上对 EP6的三维结构进行了同源模建 .计算机模型显示 EP6表面包含一个由重轻链 CDRs围成的 ,可能用于半抗原结合和催化活性的长裂缝 ,其中轻链的 CDR3(L- CDR3)和重链三个 CDRs(H- CDRs)都可能参与抗原或半抗原的接触 ,L- CDR3和 H- CDR2的贡献较大 ,对抗体酶功能可能起重要作用
The shuttle (O1-methyl-O2- (1,2,2-trimethylpropyl) -2-hydroxy-5-nitrophenylmethylphosphonic acid) The single chain antibody enzyme EP6 was used to determine the nucleotide sequence of this antibody. The sequencing results showed that the full length of this single chain antibody gene was 71.4 bp, in which the heavy chain was 351 bp long and encoded a 1-17 amino acid The chain length is 31 8 bp and encodes a protein of 106 amino acids linked by a 45 bp linker gene encoding (Gly4 Ser) 3. Both the heavy and light chains are open reading frames, with well-defined four framework regions and three complements (CDR) and two cysteine residues characteristic of the antibody, indicating that the amino acid sequences of the heavy and light chains are in accordance with the characteristics of the variable region of the mouse antibody.The homology modeling of the three-dimensional structure of the EP6 was carried out on the SGI workstation The computer model shows that the EP6 surface contains a long cleft surrounded by heavy light CDRs that may be used for hapten binding and catalytic activity, where the CDR3 (L-CDR3) of the light chain and the three CDRs of the heavy chain (H-CDRs ) May be involved in the antigen or hapten contact, the contribution of L-CDR3 and H-CDR2 larger antibody abase function may play an important role use