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目的：研究新型重组人肿瘤坏死因子α（ＮｒｈＴＮＦ－α）对鼻咽癌细胞株（ＣＮＥ－２）的细胞致死性和联合用药的协同作用。方法：用细胞毒分析法观察了ＮｒｈＴＮＦ－α、重组人肿瘤坏死因子α（ｒｈＴＮＦ－α）对ＣＮＥ－２生长的影响，并对不同联合用药模式进行研究。结果：ＮｒｈＴＮＦ－α对ＣＮＥ－２细胞的杀伤作用明显强于ｒｈＴＮＦ－α，同一浓度的杀伤作用强２～３倍。细胞杀伤效应为剂量依赖型，ＮｒｈＴＮＦ－α与重组人干扰素γ（ｒｈＩＦＮ－γ）联合有较好的协同作用；再与顺铂（ＤＤＰ）联合应用则协同作用更强。结论：ＮｒｈＴＮＦ－α对ＣＮＥ－２细胞有很强的细胞杀伤性，ｒｈＩＦＮ－γ和ＤＤＰ可明显增强ＮｒｈＴＮＦ－α的细胞毒性。
Objective: To investigate the synergistic effect of a novel recombinant human tumor necrosis factor α (NrhTNF-α) on cell death and combination therapy of nasopharyngeal carcinoma cell line (CNE-2). Methods: The effects of NrhTNF-α and rhTNF-α on the growth of CNE-2 were observed by cytotoxicity assay and the different combination modes were studied. Results: The killing effect of NrhTNF-α on CNE-2 cells was stronger than that of rhTNF-α, and the killing effect of the same concentration was 2-3 times stronger than that of rhTNF-α. The cytotoxicity was dose-dependent, and NrhTNF-α and rhIFN-γ had a good synergistic effect. When combined with cisplatin (DDP), the synergistic effect was stronger. CONCLUSION: NrhTNF-α has a strong cytotoxicity on CNE-2 cells. RhIFN-γ and DDP can significantly enhance the cytotoxicity of NrhTNF-α.