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三子合剂为中西医结合专家刘隆棣教授运用现代药理研究成果,研制出的中药复方雾化吸入剂,临床用于治疗支气管哮喘取得较好疗效,为进一步探讨其防治哮喘机理,进行了动物实验,报道如下。1 材料1.1 动物 健康幼年豚鼠,体重150~200g,共32只,雌雄各半,由南京中医药大学动物实验中心提供。1.2 实验设备 4L密闭喷雾箱、S888E型超声雾化仪(南京道芬电子有限公司产)、超速离心机(日本日立)1.3 主要试剂 卵清白蛋白:Sigma公司提供,批号:970801;苏木素—伊红(HE):由南京中医药大学门诊部实验室提供;T细胞亚群检测试剂盒:由北京军事医学
Sanzi Mixture is a combination of Chinese and Western medicine expert Professor Liu Longxi using modern pharmacological research results, developed a traditional Chinese medicine compound aerosol inhaler, clinically used in the treatment of bronchial asthma to obtain better efficacy, in order to further explore its prevention and treatment of asthma, conducted animal experiments, The report is as follows. 1 Materials 1.1 Animals Healthy juvenile guinea pigs, weighing 150-200g, a total of 32, male and female, provided by the Animal Experimental Center of Nanjing University of Traditional Chinese Medicine. 1.2 Experimental equipment 4L sealed spray box, S888E ultrasonic atomization instrument (produced by Nanjing Daofen Electronics Co., Ltd.), ultracentrifuge (Japan Hitachi) 1.3 main reagent egg albumin: provided by Sigma, batch number: 970801; hematoxylin - eosin (HE): provided by the laboratory of the outpatient department of Nanjing University of Traditional Chinese Medicine; T-cell subset detection kit: from Beijing Military Medical