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目的:研究胼酞嗪对兔脉络膜血流和激光诱导的鼠脉络膜新生血管及人脐静脉内皮细胞管状结构形成的影响。方法:雌性新西兰白兔左眼眼内压升高至40mmHg后,滴入10g/L胼酞嗪滴眼液,采用彩色微球技术测量眼血流变化。用Nd:YAG激光诱导雄性Brown Norway大鼠至Bruch膜破裂,而后分别予生理盐水或5,10和20g/L胼酞嗪滴眼液点眼,3次/d,连续4wk。采用眼底荧光血管造影和脉络膜平片测量新生血管的面积。此外亦用不同浓度的胼酞嗪作用于培养之人脐静脉内皮细胞并探讨对其管状结构形成的影响。结果:10g/L胼酞嗪滴眼液滴入眼内压为40mmHg的兔眼30,60min后,脉络膜血流明显增加。眼底荧光血管造影和脉络膜平片测量均显示,经过4wk药物治疗,5,10,20g/L胼酞嗪滴眼液均明显抑制了鼠眼CNV的形成。3～30mg/L胼酞嗪对在基质凝胶中培养了48h的人脐静脉内皮细胞的管状结构的形成有抑制作用。结论:胼酞嗪可以抑制体内脉络膜新生血管及体外培养的人脐静脉内皮细胞管状结构的形成,并增强缺血后的兔脉络膜血流。胼酞嗪有望成为治疗年龄相关性黄斑变性的药物。
OBJECTIVE: To study the effect of hydralazine on the formation of choroidal blood flow and laser-induced choroidal neovascularization and tubular structure of human umbilical vein endothelial cells. Methods: After the intraocular pressure of female New Zealand white rabbits was increased to 40mmHg, 10g / L hydralazine eye drops were dripped and the ocular blood flow changes were measured by color microspheres technique. Male Brown Norway rats were induced to rupture at Bruch’s membrane with Nd: YAG laser, and then treated with normal saline or 5, 10 and 20 g / L hydralazine eye drops for 3 times daily for 4 weeks. Fundus fluorescein angiography and choroidal flat films were used to measure the area of neovascularization. In addition, different concentrations of hydralazine were also applied to cultured human umbilical vein endothelial cells and their effects on the formation of tubular structures were also investigated. Results: 10g / L hydralazine eye drops into the intraocular pressure of 40mmHg rabbit eyes 30,60min, the choroidal blood flow increased significantly. Fundus fluorescein angiography and choroidal flat film measurements showed that after 4wk drug treatment, 5,10,20 g / L hydralazine eye drops significantly inhibited the formation of mouse eye CNV. The effect of 3 ~ 30mg / L hydralazine on the formation of tubular structure of human umbilical vein endothelial cells incubated in matrix gel for 48h. Conclusion: Hydralazine can inhibit the formation of tubular structures in human choroidal neovascularization and cultured human umbilical vein endothelial cells and enhance the choroidal blood flow in rabbits after ischemia. Hydralazine is expected to become a drug for the treatment of age-related macular degeneration.