论文部分内容阅读
目的肝细胞对TGF-β1敏感性的丧失据认为是肝癌重要的致病因素之一。本研究旨在明确人肝肿瘤细胞系中TGF-β1的作用及其与凋亡的关系。方法选用三种含不同p53基因状态的人肝肿瘤细胞系,应用脱氧核糖核苷酸末瑞转移酶介导的dUTP缺口末端标记技术(TUNEL)对TGF-β1诱导的肝肿瘤细胞的凋亡进行定量检测。结果在应用TUNEL检测三个细胞系中,TGF-β1仅能诱导Hep3B细胞(缺失p53)则凋亡较少。这提示凋亡p53基因的表达具有明确的联系。结论HepG2细胞系比Huh-7和Hep3B系细胞更易发生TGF-β1诱导的凋亡,TGF-β1通过p53依赖性途径诱导肝癌细胞系发生凋亡。
The loss of susceptibility of TGF-β1 by target liver cells is considered to be one of the important pathogenic factors of liver cancer. This study aimed to clarify the role of TGF-β1 in human liver tumor cell lines and its relationship with apoptosis. Methods Three human liver tumor cell lines with different p53 gene status were selected. TUNEL was used to induce apoptosis of liver tumor cells induced by TGF-β1 using a deoxyribonucleotide-enduitase-mediated dUTP nick end labeling technique (TUNEL). Quantitative testing. Results In the three cell lines detected by TUNEL assay, TGF-β1 only induced Hep3B cells (deletion of p53) and less apoptosis. This suggests a clear link between the expression of apoptotic p53 genes. Conclusion HepG2 cell line is more likely to undergo TGF-β1-induced apoptosis than Huh-7 and Hep3B cells, and TGF-β1 induces apoptosis of hepatoma cell lines through a p53-dependent pathway.