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为明确 Rubisco活化酶在叶片衰老期间是否对 Rubisco羧化活性和光合速率起调节作用 ,本文研究了水稻剑叶衰老过程中叶片光合速率、可溶性蛋白含量、Rubisco初始羧化活力及含量 ,Rubisco活化酶活力及含量 .结果表明 :净光合速率与 Rubisco初始羧化活力和 Rubisco活化酶的活力间分别为 r2=0 .9663和 r2 =0 .70 2 ,Rubisco初始羧化活力与 Rubisco活化酶的活力和含量间分别为 r2 =0 .81 0 3和r2 =0 .81 4 3 ,Rubisco活化酶含量与 Rubisco和可溶性蛋白间分别为 r2 =0 .92 57和 0 .8675,Rubisco活化酶的活力与含量间 r2 =0 .7587.Rubisco活化酶的含量占 Rubisco含量的 0 .5%～ 1 .0 % ,占叶片可溶性总蛋白质的 0 .5%～ 0 .6% ,随着剑叶衰老 Rubisco活化酶所占的比值加速下降 .这表明水稻剑叶衰老期间 ,Rubisco活化酶对维持 Rubisco初始羧化活力和净光合速率有重要调节作用 .
In order to determine whether Rubisco activase plays a regulatory role on the carboxylation activity and photosynthetic rate of Rubisco during leaf senescence, the leaf photosynthetic rate, soluble protein content, Rubisco initial carboxylation activity and content, Rubisco activase Vitality and content of Rubisco.The results showed that the initial photosynthetic rate of Rubisco and the Rubisco activase activity were r2 = 0.9663 and r2 = 0.702 respectively.The initial carboxylation activity of Rubisco and the activity of Rubisco activase and The contents of Rubisco activase and Rubisco and soluble protein were r2 = 0.92 57 and 0.8675, respectively. The activity and content of Rubisco activase R2 = 0.7587.Rubisco enzyme content accounted for 0.5% ~ 1.0% of Rubisco content, total leaf soluble protein 0.5% ~ 0.6%, with the flag leaf aging Rubisco activase , Indicating that Rubisco activase plays an important role in maintaining the initial carboxylation viability and net photosynthetic rate of Rubisco during senescence of rice flag leaf.