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用微生物回复突变试验、体外培养人淋巴细胞染色体畸变试验和小鼠骨髓多染红细胞微核试验三种方法,研究N′—乙酰靛玉红致突变作用。结果发现无论加入或不加入肝微粒体酶活化,0.025～250μg/片及0.25～2500μg/皿剂量的N′—乙酰靛玉红,对鼠伤寒沙门氏菌组氨酸缺陷型菌株均无致基因突变性;25～75μg/ml的N′—乙酰靛玉红均不能诱发体外培养人淋巴细胞染色体畸变;1250～5000mg/kg的N′—乙酰靛玉红对小鼠骨髓细胞染色体亦无致畸变作用。
The mutagenicity of N′-acetyl indirubin was studied using the methods of microbial revert mutation test, in vitro culture of human lymphocyte chromosomal aberration test and mouse bone marrow polychromatic erythrocyte micronucleus test. The results showed that N′-acetyl indirubin at doses of 0.025 to 250 μg/tablet and 0.25 to 2500 μg/dish did not cause mutations in the histidine-deficient strains of Salmonella typhimurium, with or without the addition of hepatic microsomal enzymes. 25-75 μg/ml of N′-acetyl indirubin could not induce chromosomal aberration in human lymphocytes cultured in vitro; N′-acetyl indirubin 1250-5000 mg/kg also had no aberration effect on chromosomes of bone marrow cells in mice.