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目的:在模拟高原缺氧条件下建立稳定的缺氧SD大鼠牙周炎动物模型。方法:40只SD大鼠,雌雄各半,采用随机数字表法分为4组:常氧对照组(A组),常氧牙周炎组(B组),缺氧对照组(C组),缺氧牙周炎组(D组),每组10只。B、D组大鼠结扎牙颈部、高糖饮食、口腔涂菌及注射激素,A、C组不作任何处理,正常饮食。C、D组喂养的同时进入模拟海拔5000 m的低压氧舱。8 w后检测牙龈指数GI、菌斑指数PLI、牙周袋深度PD、牙槽骨吸收度ABL及组织病理学观察,对模型进行评估。结果:GI、PD及ABL指数在B组与A组,D组与C组,C组与A组,D组与B组比较均有统计学差异(P<0.05)。PLI指数在B组与A组,D组与C组比较有统计学差异(P<0.05),在C组与A组,D组与B组比较无统计学差异(P>0.05)。HE染色见A、C组无明显改变,B、D组牙龈糜烂,结合上皮向根方增殖迁移,牙周膜间隙增宽。结论:将采用结扎牙颈部、高糖饮食、口腔涂菌及注射激素综合方法作用的SD大鼠放入模拟海拔5000 m的低压氧舱,建立模拟高原缺氧大鼠牙周炎模型具有可行性,为高原牙周病的防治奠基了实验基础。
OBJECTIVE: To establish a stable animal model of periodontitis in SD rats under simulated hypoxia. Methods: Forty SD rats were randomly divided into 4 groups: normoxic control group (A group), normoxia periodontitis group (B group), hypoxic control group (C group) , Hypoxia periodontitis group (group D), 10 rats in each group. Rats in group B and group D were ligated with high-sugar diet, oral cavity and injecting hormone. Rats in groups A and C received no treatment and were given normal diet. Group C and D were fed into hypobaric chamber simulating altitude 5000 m at the same time. After 8 weeks, gingival index (GI), plaque index (PLI), periodontal pocket depth (PD), alveolar bone resorption (ABL) and histopathology were measured to evaluate the model. Results: The indexes of GI, PD and ABL were significantly different between group B and group A, group D and group C, group C and group A, group D and group B (P <0.05). PLI index was significantly different between group B and group A, group D and group C (P <0.05). There was no significant difference between group C and group A, group D and group B (P> 0.05). HE staining, A, C group had no significant change, B, D group gingival erosion, combined with epithelial proliferation and migration to the root, periodontal membrane gap widened. CONCLUSION: It is feasible to establish a hypoxic rat model with periodontitis by altering the neck of rats, high-sugar diet, oral dip-coating and injecting hormones into the hypoxic chamber at an altitude of 5000 m Sex, for the prevention and treatment of periodontal disease laid the foundation for the experiment.