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目的:探讨黄体生成素受体(LHR)及类固醇激素合成急性调节蛋白(StAR)在大鼠多囊卵巢综合征(PCOS)模型中的表达及作用。方法:将50只21日龄SD大鼠随机分为模型组(PCOS组,n=35)和对照组(NC组,n=15)。通过注射脱氢表雄酮(DHEA)建立PCOS大鼠模型,放射免疫法和酶联免疫法分别检测血清孕酮(P)和雌二醇(E_2)水平及睾酮(T)水平;免疫组织化学法对LHR及StAR进行细胞定位分析,RT-PCR和Western blotting分别检测LHR及StAR mRNA和蛋白表达变化。结果:与NC组相比,血清E_2和T水平显著升高,P水平无显著变化;LHR和StAR mRNA和蛋白表达水平均显著升高。结论:PCOS高雄激素血症可能与LHR和StAR表达调控有关;两者与E_2水平升高之间的关系仍有待阐明。
Objective: To investigate the expression and function of luteinizing hormone receptor (LHR) and steroidogenic acute regulatory protein (StAR) in rat polycystic ovary syndrome (PCOS) model. Methods: Fifty 21-day-old SD rats were randomly divided into model group (PCOS group, n = 35) and control group (NC group, n = 15). PCOS rat model was established by injecting dehydroepiandrosterone (DHEA). Serum progesterone (P) and estradiol (E2) and testosterone (T) levels were detected by radioimmunoassay and enzyme-linked immunosorbent assay. Methods LHR and StAR cell localization analysis, RT-PCR and Western blotting were detected LHR and StAR mRNA and protein expression changes. Results: Compared with NC group, the levels of E_2 and T in serum increased significantly and the levels of P did not change significantly. The mRNA and protein expressions of LHR and StAR were significantly increased. Conclusion: Hyperandrogenism in PCOS may be related to the regulation of LHR and StAR expression. The relationship between them and the increase of E_2 level remains to be elucidated.