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A modified method of preparing 20(S)-ginsenoside Rh_2(G-Rh_2) and the inhibitory effect of 20(S)-ginse-noside Rh_2 on Hep-A-22 cells were investigated.The total saponins and strong alkali were dissolved in glycerol at the atmospheric pressure,and the degradation was performed at a high temperature.After G-Rh_2 had been isolated and purified,MTT(methyl thiazolyl tetrazolium) assay was applied to evaluating the effect of 20(S)-ginsenoside Rh_2 on the cells viability and morphological changes were observed.It was shown that 20(S)-ginsenoside Rh_2 can reduce Hep-A-22 cells viability in dose-dependent manner and the cells took on cell shrinkage,membrane blebbing,chromosomal condensations,especially under the higher concentrations of it.In conclusion,20(S)-ginsenoside Rh_2 can be prepared effectively that not only decreases viability but also induces the apoptosis of Hep-A-22 cells.
A modified method of preparing 20 (S) -ginsenoside Rh_2 (G-Rh_2) and the inhibitory effect of 20 (S) -ginse-noside Rh_2 on Hep-A-22 cells were investigated.The total saponins and strong alkali were dissolved in glycerol at the atmospheric pressure, and the degradation was performed at a high temperature. After G-Rh_2 had been isolated and purified, MTT (methyl thiazolyl tetrazolium) assay was applied to evaluating the effect of 20 (S) -ginsenoside Rh_2 on the cells viability and morphological changes were observed. It was shown that 20 (S) -ginsenoside Rh_2 can reduce the viability of Hep-A-22 cells in dose-dependent manner and the cells took on cell shrinkage, membrane blebbing, chromosomal condensations, especially under the higher concentrations of it.In conclusion, 20 (S) -ginsenoside Rh_2 can be prepared effectively that not only reduces viability but also induces the apoptosis of Hep-A-22 cells.