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目的探讨卡介苗(BCG)对哮喘小鼠气道炎症、T_H细胞分化及血清OVA特异性IgE的影响。方法动物随机分为阴性对照组、OVA致敏激发组和BCG干预组。检测气管血管旁嗜酸性粒细胞(EOS)数目、气管上皮杯状细胞增生指数和黏液储存指数、小鼠外周血上清液中细胞因子白细胞介素5(IL-5)及γ干扰素(IFN-γ)浓度、小鼠肺组织中T-bet和GATA-3 mRNA水平及小鼠血清中OVA特异性IgE表达。结果BCG干预组和OVA致敏激发组中小鼠肺气管血管旁单位面积EOS数目分别为(65.09±32.58)个/mm~2、(941.86±592.03)个/mm~2,两者比较差异有统计学意义(P<0.05);BCG干预组和OVA致敏激发组杯状细胞增生指数分别为(19.25±12.27)个/mm、(54.39±24.50)个/mm,BCG干预组和OVA致敏激发组黏液储存指数分别为(6.47±3.94)%、(15.53±11.38)%,两者比较差异有统计学意义(P<0.001);BCG干预组和OVA致敏激发组IL-5浓度分别为(83.40±11.33)pg/ml、(192.30±44.30)pg/ml,IFN-γ浓度分别为(28.69±6.05)pg/ml、(18.12±3.78)pg/ml,T-bet/GATA-3 mR- NA比值分别为1.02±0.18、0.74±0.07,两者比较均差异有统计学意义(P<0.01);BCG干预组和OVA致敏激发组血清中OVA特异性IgE的表达分别为0.61±0.27、1.05±0.45,两者比较差异有统计学意义(P<0.05)。结论BCG能抑制哮喘小鼠气道EOS浸润和黏液高分泌状态,影响T-bet/GATA-3表达水平,降低IL-5水平而增高IFN-γ水平,并能抑制哮喘小鼠血清中OVA特异性IgE的表达。
Objective To investigate the effects of BCG on airway inflammation, T-H cell differentiation and serum OVA-specific IgE in asthmatic mice. Methods Animals were randomly divided into negative control group, OVA sensitized challenge group and BCG intervention group. The numbers of eosinophils (EOS), tracheal epithelial goblet cell hyperplasia index and mucus storage index in trachea were detected. The levels of interleukin 5 (IL-5), IFN-γ -γ), T-bet and GATA-3 mRNA levels in mouse lung tissue and OVA-specific IgE expression in mouse serum. Results The numbers of EOS per unit area of lung tracheal peritoneum were (65.09 ± 32.58) / mm ~ 2 and (941.86 ± 592.03) / mm ~ 2 respectively in BCG intervention group and OVA sensitized challenge group, there were statistically significant differences (19.25 ± 12.27) / mm, (54.39 ± 24.50) / mm respectively in BCG intervention group and OVA-sensitized challenge group, BCG intervention group and OVA sensitization challenge (6.47 ± 3.94)% and (15.53 ± 11.38)%, respectively, the difference between the two groups was statistically significant (P <0.001). The concentrations of IL-5 in BCG intervention group and OVA sensitized group were 83.40 ± 11.33 pg / ml and 192.30 ± 44.30 pg / ml respectively, the IFN-γconcentrations were (28.69 ± 6.05) pg / ml, NA ratio were 1.02 ± 0.18 and 0.74 ± 0.07, respectively (P <0.01). The serum levels of OVA-specific IgE in BCG intervention group and OVA-sensitized challenge group were 0.61 ± 0.27, 1.05 ± 0.45, the difference was statistically significant (P <0.05). Conclusion BCG can inhibit EOS infiltration and mucosal hypersecretion in asthmatic mice, affect the expression of T-bet / GATA-3, decrease the level of IL-5 and increase the level of IFN-γ in asthmatic mice, and also inhibit the serum level of OVA in asthmatic mice Sex IgE expression.