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目的 探讨哮喘儿童血白细胞介素 -5 (IL-5 )和嗜酸细胞阳离子蛋白 (ECP)含量变化及其关系。方法 采用双抗夹心 ELISA法和 pharmacia CAP检测系统对 85例哮喘患儿急性发作期及治疗后血清 IL-5和 ECP水平进行检测。结果 哮喘发作期血清 IL-5 (65 8.97± 3 60 .0 1 ) pg/ml和 ECP(2 5 .0 3± 2 2 .3 4)μg/L ;治疗后 IL-5 (3 2 7.92±2 46.3 6) pg/ml,ECP(9.84± 8.48μg/L) ,治疗前后比较差异有非常显著性 (P <0 .0 1 )。哮喘发作期及缓解期血清中 IL-5和 ECP两者之间存在显著正相关急性期 r=0 .5 42 6,P <0 .0 1 ;缓解期 r=0 .3 80 5 ,P <0 .0 5。结论 IL-5和 ECP参与哮喘的发生发展 ,在哮喘发病机制中起重要作用
Objective To investigate the changes of serum interleukin-5 (IL-5) and eosinophil cationic protein (ECP) in children with asthma and their relationship. Methods Serum levels of IL-5 and ECP were measured in 85 asthmatic children during acute exacerbation and after treatment with double-antibody sandwich ELISA and pharmacia CAP detection system. Results Serum levels of IL-5 (P <0.05) and ECP (25.3 ± 22.44 μg / L) were significantly higher in the asthmatic group than those in the control group 2 46.3 6) pg / ml, and ECP (9.84 ± 8.48 μg / L). There was a significant difference between before and after treatment (P <0.01). There was a significant positive correlation between serum IL-5 and ECP during the onset and remission of asthma. R = 0.542 6, P <0.01; remission r = 0.385, P < 0 .0 5. Conclusion IL-5 and ECP are involved in the development of asthma and play an important role in the pathogenesis of asthma