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目的:通过建立大强度耐力训练及一次力竭性游泳运动损伤模型,研究罗汉果叶黄酮抗疲劳能力以及维持细胞膜内外的渗透压及跨膜电位平衡中的作用。方法:选用健康雄性SD大鼠40只,2～3月龄,体重180～220 g,随机分成5组,每组8只,即:安静对照组、安静力竭组、安静给药力竭组、训练力竭组、训练+给药力竭组。给药量以生理盐水配成含罗汉果黄酮提取物200 mg.kg-1.d-1(20 g.L-1的溶液,按10.0 mL.kg-1.d-1ig给药),对照组ig等量的生理盐水。在递增负荷游泳训练4周后,各组进行3%的负重力竭性游泳训练,准确记录力竭时间后即刻断头取血(安静对照组同时取血);测定股四头肌的三磷酸腺苷(ATP)酶(ATPase)相关指标的含量。结果:力竭运动后即刻,Na+/K+-ATP酶活性除训练给药组外均极显著下降(P<0.01),训练给药组显著高于其他力竭组,与安静对照组相比无显著性差异。Ca2+-ATP酶活性在力竭运动后即刻也呈现下降趋势,除训练给药组外均显著低于安静对照组,而给药组和训练组又显著高于安静力竭组(P<0.05,P<0.01),且呈上升趋势,但这3组间数据差异均无统计学意义。力竭运动后即刻,股四头肌中总ATP酶活性显著下降,但训练组和给药组均极显著高于安静力竭组(P<0.01),其中训练给药组显著高于安静给药力竭组和训练力竭组(P<0.01)。结论:服用罗汉果叶黄酮可提高游泳至力竭后大鼠股四头肌Na+/K+-ATPase,Ca2+/Mg2+-ATPase及总ATPase活力,提示罗汉果叶黄酮对维持细胞膜内外的渗透压及跨膜电位平衡具有重要意义,同时还能维持细胞内外Ca2+/Mg2+的稳态,保持较高的能量供给,提高抗氧化能力,延缓运动疲劳的发生。
OBJECTIVE: To establish an intensive endurance training and an exhaustive swimming injury model to study the anti-fatigue ability of Luohanguo flavonoids and the role of osmotic pressure and transmembrane potential balance in and outside the cell membrane. Methods: Forty healthy male Sprague Dawley rats aged 2 ~ 3 months and weighing 180 ~ 220 g were randomly divided into 5 groups with 8 rats in each group: Quiet control group, Quiet exhaust group, Quiet administration Exhaustion group , Training exhaustion group, training + drug exhaust group. Dosage with saline, containing Luohanguo flavonoids extract 200 mg.kg-1.d-1 (20 gL-1 solution, according to 10.0 mL.kg-1.d-1ig administration), the control group ig The amount of saline. After 4 weeks of incremental load swimming training, 3% weight-bearing exhaustive swimming training was carried out in each group, and the blood was collected immediately after exhaustive time (quiet control group at the same time taking blood); Determination of quadriceps triphosphate adenosine triphosphate ATP) enzyme (ATPase) related indicators. Results: Immediately after exhaustive exercise, Na + / K + -ATPase activity decreased significantly (P <0.01) except for training group, and was significantly higher in training group than in other exhaustive group Significant difference. Ca2 + -ATPase activity also showed a downward trend immediately after exhaustive exercise, except for the training and administration groups were significantly lower than the quiet control group, while the administration group and training group was significantly higher than the quiet exhaustion group (P <0.05, P <0.01), and showed an upward trend, but there was no significant difference between the three groups in data. Immediately after exhaustive exercise, the total ATPase activity in the quadriceps decreased significantly, but the training group and the administration group were significantly higher than those in the quiet exhaustion group (P <0.01), and the training group was significantly higher than the rest group Exhaustion group and training exhaustion group (P <0.01). Conclusion: Taking Luohanguo leaf flavonoids can improve the activity of Na + / K + -ATPase, Ca2 + / Mg2 + -ATPase and total ATPase in the quadriceps femoris after swimming to exhaustion, suggesting that the flavonoids from Luohaoguo could maintain the osmotic pressure and transmembrane potential Balance is important, while maintaining intracellular and extracellular Ca2 + / Mg2 + homeostasis, to maintain a high energy supply, improve antioxidant capacity, delay the occurrence of exercise-induced fatigue.