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目的：自体血栓注入法建立急性肺栓塞动物模型，探讨内皮素在急性血栓栓塞性肺动脉高压中的作用和溶栓疗法对肺动脉释放内皮素的影响。方法：14只杂种犬，自体血栓注入法建立急性肺栓塞模型。模型犬随机分入溶栓组和对照组，溶栓组7只犬给予尿激酶20000U/kg，100ml液体稀释后30分钟静脉滴入，对照组同期输入等量的生理盐水。分别于注栓前、溶栓前（对照组同期）、溶栓后（对照组同期）血液动力学测定，采动脉血放免法测定血浆内皮素水平。结果：溶栓组犬静注尿激酶30分钟后，伴随肺动脉压力和肺血管阻力的显著性下降，动脉血浆内皮素水平显著升高，溶栓后30小时为99.25±12.90pg/L，与注栓前51．63±10．09pg/L比较P＜005，与对照组同期值55．93±2.10pg/L比较P＜001，对照组犬栓塞后4小时内肺动脉压力和肺血管阻力无显著变化，内皮素水平亦无显著性改变。结论：内皮素可能与急性血栓栓塞性肺动脉高压的形成有关，动脉血浆内皮素水平的增加可能是血管再通的一个标志。
OBJECTIVE: To establish an animal model of acute pulmonary embolism by autogenous thrombectomy to investigate the role of endothelin in acute thromboembolic pulmonary hypertension and the effect of thrombolytic therapy on the release of endothelin in pulmonary arteries. Methods: Fourteen crossbred dogs were induced by autologous thrombosis to establish an acute pulmonary embolism model. The model dogs were randomly divided into thrombolytic group and control group. Thrombolysis group 7 dogs were given urokinase 20000U / kg, 100ml liquid diluted 30 minutes after intravenous infusion, the same period the control group, the same amount of saline. The levels of plasma endothelin were measured before pretreatment, before thrombolysis (in the same period as the control group) and after thrombolysis (in the same period as the control group), respectively. Results: Thrombolytic group, 30 minutes after intravenous injection of urokinase, accompanied by a significant decrease in pulmonary artery pressure and pulmonary vascular resistance, arterial plasma endothelin levels were significantly increased, 30 hours after thrombolysis was 99.25 ± 12.90pg / L, and injection In the control group, pulmonary arterial pressure and pulmonary vascular resistance were not significantly elevated within 4 hours after embolization in the control group, compared with 51.63 ± 10.09pg / L in the pre-suppository group, P <005, and P <001 in the same period as the control group (55.93 ± 2.10pg / L) Changes, endothelin levels also did not change significantly. Conclusion: Endothelin may be related to the formation of acute thromboembolic pulmonary hypertension. The increase of plasma endothelin levels may be a sign of recanalization.