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目的对高危型人乳头瘤病毒(Human papillomavirus)16型E6蛋白的B细胞线性表位进行预测。方法以HPV16型E6蛋白(P03126)的全长氨基酸序列为基础,采用EXPASY网站上的生物信息学软件和DNAstar软件包中的Protean软件分析E6蛋白的二级结构,跨膜趋势及其亲水性,表面可及性,抗原性,极性和柔韧性等特性。综合以上参数预测HPV16型E6蛋白的B细胞线性表位,并进一步进行同源性匹配分析。结果综合多个参数分析显示B细胞线性表位可能位于E6蛋白N端2-7、9-19、30-36、45-49、105-109、119-125和148-158区段及其附近,进一步同源性匹配分析支持其中区段2-7、9-19、119-125及148-158为可能的B细胞线性表位,其中区段148-158(RSSRTRRETQL)为HPV16型E6蛋白所特有的序列,而区段2-7(HQKRTA)、9-19(FQDPQERPRKL)与HPV9型E6蛋白具有同源性,区段119-125(PEEKQRH)与HPV31型E6蛋白具有同源性。结论综合分析预测HPV16型E6蛋白的B细胞线性表位可能位于E6蛋白N端2-7、9-19、119-125及148-158区段,为HPV16型E6蛋白的进一步研究奠定了基础。
Objective To predict the linear B cell epitopes of human papillomavirus type 16 E6 protein. Methods Based on the full-length amino acid sequence of HPV16 E6 protein (P03126), bioinformatics software from EXPASY and Protean software from DNAstar software package were used to analyze the secondary structure, transmembrane trend and hydrophilicity of E6 protein , Surface accessibility, antigenicity, polarity and flexibility. Based on the above parameters, B cell linear epitopes of HPV16 E6 protein were predicted and further analyzed by homology matching. Results The analysis of multiple parameters showed that B-cell linear epitopes may be located in the N-terminal 2-7, 9-19, 30-36, 45-49, 105-109, 119-125 and 148-158 segments of E6 protein and its vicinity , Further homology matching analysis supports the linear B-cell epitopes in which segments 2-7, 9-19, 119-125 and 148-158 are possible, wherein segment 148-158 (RSSRTRRETQL) is HPV16 type E6 protein While segments 2-7 (HQKRTA), 9-19 (FQDPQERPRKL) have homology with HPV9 E6 protein, and segments 119-125 (PEEKQRH) have homology with HPV31 E6 protein. Conclusion A comprehensive analysis predicts that the B cell linear epitope of HPV16 E6 protein may be located in the N-terminal 2-7, 9-19, 119-125 and 148-158 segments of E6 protein, which lays a foundation for further study of HPV16 E6 protein.