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目的:观察大鼠脑缺血预处理后胶质纤维酸性蛋白(glial bifilarly acidic protein,GFAP)、神经生长因子(nerve growth factor,NGF)、脑源性神经营养因子(brain derived neurotrophic factor,BDNF)的表达及对神经功能的影响。方法:将大鼠随机分为正常组、假手术组、脑缺血再灌注模型组(ischemia reperfusion group,IR)及脑缺血预处理组(ischemic preconditioning group,IP),于缺血后2 h及再灌注后各个时间点进行神经功能缺损评分,比较各组脑梗死体积的大小。通过苏木精-伊红(hematoxylin-eosin,HE)染色观察梗死区脑组织细胞的形态学改变,利用免疫组织化学方法检测梗死区组织GFAP、NGF、BDNF的表达变化。结果:与IR组相比,IP组在术后的神经功能缺损评分明显降低,梗死体积明显减少(P<0.05),各个时间点的GFAP、NGF、BDNF表达量明显上调(P<0.05),差异具有统计学意义。结论:缺血预处理减轻脑缺血再灌注损伤可能与神经再生及分化有关。
Objective: To observe the effects of glial bifilarly acidic protein (GFAP), nerve growth factor (NGF), brain derived neurotrophic factor (BDNF) Expression and the impact on neurological function. Methods: The rats were randomly divided into normal group, sham operation group, ischemia reperfusion group (IR) and ischemic preconditioning group (IP). After 2 h of ischemia The neurological deficit score was measured at various time points after reperfusion, and the infarct volume of each group was compared. The morphological changes of infarcted brain tissue were observed by hematoxylin-eosin (HE) staining. The expression of GFAP, NGF and BDNF in infarcted area were detected by immunohistochemistry. Results: Compared with IR group, the score of neurological deficit in IP group decreased significantly and infarct volume decreased significantly (P <0.05). The expression of GFAP, NGF and BDNF at each time point was significantly increased (P <0.05) The difference was statistically significant. CONCLUSION: Ischemic preconditioning attenuates cerebral ischemia-reperfusion injury and may be related to nerve regeneration and differentiation.