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为了研究巴西橡胶树中植物特有NAC转录因子的功能,笔者通过RT-PCR和实时荧光定量PCR技术对HbNAM的开放阅读框(ORF)进行了克隆和表达分析,并通过酵母转化实验进行转录激活活性分析。结果显示,HbNAM基因ORF为1 077 bp,编码358个氨基酸,在第12—167位氨基酸之间含有一个典型的NAC结构域,属于NAC转录因子家族中的NAM亚族。酵母试验表明,HbNAM在高度变异C端具有转录激活活性,具备转录因子的基本特征。实时荧光定量PCR分析发现,HbNAM在叶片、雄花、雌花、胶乳和树皮中均表达,其中以光合组织叶片和生殖组织雄花中的表达量最高,并且胶乳中该基因的表达受割胶、植物激素或植物生长调节剂茉莉酸(JA)和乙烯利(ET)以及低温胁迫影响,推测HbNAM可能与植物的生长发育和胁迫应答过程有关。
In order to study the function of plant-specific NAC transcription factors in Tamarix, we cloned and expressed the open reading frame (ORF) of HbNAM by RT-PCR and real-time fluorescence quantitative PCR and analyzed the transcriptional activation activity by yeast transformation . The results showed that the ORF of HbNAM gene was 1 077 bp, encoding 358 amino acids. It contained a typical NAC domain between amino acids 12-167 and belongs to the NAM subfamily of NAC transcription factor families. Yeast tests showed that HbNAM has a transcriptional activation activity at the highly variable C-terminal and possesses the basic characteristics of a transcription factor. Real-time quantitative PCR analysis showed that HbNAM was expressed in leaves, male flowers, female flowers, latex and bark, among which the highest expression was in photosynthetic and reproductive male flowers, and the expression of the gene in latex was affected by tapping, Or plant growth regulators jasmonic acid (JA) and ethephon (ET) as well as the effects of low temperature stress, speculated that HbNAM may be related to plant growth and stress response process.