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目的研究外源性一氧化氮(NO)对前列腺癌细胞株生长的影响及作用机制。方法以硝普钠(SNP)为一氧化氮供体,不同浓度的SNP作用前列腺癌细胞,采用MTT法测细胞的生存率,Tunel法和流式细胞仪检测细胞的凋亡情况,通过RT-PCR法检测细胞p21waf1/cip1基因的表达。结果前列腺癌细胞生存率与低浓度的SNP成正比,而与高浓度的SNP成反比。高浓度的SNP可作用于细胞的G1期,诱导细胞凋亡,p21waf1/cip1的表达也随之上调。结论低浓度的SNP促进前列腺癌细胞的生长;高浓度的SNP促进细胞的凋亡,p21waf1/cip1的上调可能是高浓度的SNP诱导前列腺癌细胞凋亡的机制之一。
Objective To study the effect of exogenous nitric oxide (NO) on the growth of prostate cancer cell lines and its mechanism. Methods Nitroprusside (SNP) was used as a nitric oxide donor. Prostate cancer cells were treated with different concentrations of SNP. Cell viability was measured by MTT assay. Cell apoptosis was detected by Tunel method and flow cytometry. PCR method was used to detect the expression of p21waf1 / cip1 gene. Results The survival rate of prostate cancer cells was proportional to the low concentration of SNPs and inversely proportional to the high concentration of SNPs. High concentration of SNP can act on the cell phase G1, induce apoptosis, p21waf1 / cip1 expression also increased. Conclusion Low concentration of SNP can promote the growth of prostate cancer cells. High concentration of SNP can promote cell apoptosis. Upregulation of p21waf1 / cip1 may be one of the mechanisms of apoptosis induced by high concentration of SNP in prostate cancer cells.