SIMPLE METHOD OF PUNCHING AND RE-LOCATING TISSUES FOR MANUAL CONSTRUCTION OF TISSUE MICROARRAY

来源 :Journal of Shanghai Second Medical University(Foreign Langua | 被引量 : 0次 | 上传用户:qq774257837
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A series of human tissue samples and cultured cell lines were formalin-fixed and paraffin-embedded. Specimen cylinder (1.2-1.8mm) were punched by a modified bone marrow biopsy needle and arrayed on a recipient paraffin block. Microscopic analysis on the sections from this tissue microarray (TMA) block demonstrated that the spots of tissues and cells were well preserved, and the cultured cell samples were successfully embedded from 5×10 4 to 2×10 5 in number. These TMA sections were also suitable for immunohistochemistry and RNA in situ hybridization. A series of human tissue samples and cultured cell lines were formalin-fixed and paraffin-embedded. Specimen cylinders (1.2-1.8mm) were punched by a modified bone marrow biopsy needle and arrayed on a recipient paraffin block. Microscopic analysis on the sections from This tissue microarray (TMA) block was demonstrated in the spots of tissues and cells were well preserved, and the cultured cell samples were successfully embedded from 5 × 10 4 to 2 × 10 5 in number. These TMA sections were also suitable for immunohistochemistry and RNA in situ hybridization.
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