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目的:探讨肥大细胞及蛋白酶激活受体-2(protease activated receptor-2,PAR-2)在肠易激综合征(irritable bowel syndrome,IBS)中的作用.方法:2012-01/2012-12自南京医科大学附属南京医院消化内科门诊随机选取腹泻型IBS患者60例及正常者30例,采用免疫组织化学检测各组肥大细胞、PAR-2蛋白的表达强度;采用Western blot检测各组Occludin的表达量.结果:免疫组织化学法结果显示IBS-D组与对照组相比,肥大细胞表达明显增多(5.20±2.78vs 1.40±0.55,P<0.05);PAR-2蛋白的表达明显增多(3.20±1.64 vs 1.20±0.45,P<0.05);Western blot结果示IBS-D组Occludin蛋白的表达明显低于正常组(2108.33±59.58 vs 3113.00±77.74,P<0.01).结论:本研究提示IBS-D患者肥大细胞过表达、激活后可刺激PAR-2活化,从而进一步损伤细胞间紧密连接.
Objective: To investigate the role of mast cells and protease activated receptor-2 (PAR-2) in irritable bowel syndrome (IBS) .Methods: Nanjing University of Medical Sciences Nanjing Hospital, Department of Gastroenterology, 60 patients were randomly selected diarrhea IBS and 30 cases of normal, using immunohistochemistry to detect the expression of mast cells and PAR-2 protein in each group; Western blot was used to detect the expression of Occludin Results: The results of immunohistochemistry showed that the expression of mast cells in IBS-D group was significantly higher than that in control group (5.20 ± 2.78 vs 1.40 ± 0.55, P <0.05), and the expression of PAR-2 protein was significantly increased 1.64 vs 1.20 ± 0.45, P <0.05). Western blot results showed that the expression of Occludin in IBS-D group was significantly lower than that in normal group (2108.33 ± 59.58 vs 3113.00 ± 77.74, P <0.01) .Conclusion: Mast cells in patients with overexpression, activation can stimulate PAR-2 activation, thereby further damage the tight junction between cells.