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为评价干扰素联合病毒唑的抗病毒效果，用套式反转录－聚合酶链反应技术观察其对慢性丙型肝炎患者正、负链丙型肝炎病毒ＲＮＡ（ＨＣＶＲＮＡ）的作用。结果显示，在对联合抗病毒治疗有反应的患者中，多数患者ＡＬＴ降至正常，血清正链阳性率由９２．３１％降至３８．４６％（Ｐ＜０．００５），周围血单核细胞（ＰＢＭＣ）中负链阳性率由７６．９２％降至３８．４６％（Ｐ＜０．０５），但对ＰＢＭＣ中正链ＨＣＶＲＮＡ影响不大。治疗停止后，半数以上患者病情复发，病情复发者均为ＰＢＭＣ中正链及负链ＨＣＶＲＮＡ持续阳性者。提示单纯检测血清正链ＨＣＶＲＮＡ作为疗效考核及病情转归的判断指标有一定的局限性。同时检测血清及ＰＢＭＣ中正、负链ＨＣＶＲＮＡ，具有重要的临床意义。本研究干扰素联合病毒唑的抗病毒效果与单用干扰素接近，似说明病毒唑并未增加干扰素的疗效
To evaluate the antiviral effect of interferon in combination with ribavirin, the effect of interferon plus ribavirin on positive and negative hepatitis C virus RNA (HCVRNA) in patients with chronic hepatitis C was examined by nested reverse transcription-polymerase chain reaction. The results showed that in patients with response to combined antiviral therapy, the majority of patients with ALT dropped to normal, positive serum positive chain rate decreased from 92.31% to 38.46% (P <0.005), peripheral blood mononuclear The positive rate of negative strand in PBMC decreased from 76.92% to 38.46% (P <0.05), but had little effect on the positive strand HCV RNA in PBMC. After the treatment was stopped, more than half of patients relapsed, the recurrence of those who are PBMC positive and negative chain HCVRNA persistent positive. Prompt detection of serum positive strand of HCVRNA as a curative effect assessment and prognosis of disease prognosis indicators have some limitations. Simultaneous detection of positive and negative HCVRNA in serum and PBMC has important clinical significance. This study of interferon and ribavirin antiviral effect and single interferon close, similar to ribavirin did not increase the efficacy of interferon