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目的:建立保护高糖损伤内皮祖细胞的丹参药渣水煎液部位的质量控制方法。方法:采用薄层色谱法(TLC),对丹参素钠、丹酚酸B进行定性鉴别。采用高效液相色谱法(HPLC)测定丹参药渣水煎液部位中丹参素钠和丹酚酸B的含量。对10批次不同时间制备的丹参药渣水煎液部位进行测定,建立了丹参药渣水煎液部位标准指纹谱,并计算各批次的相似度。结果:TLC专属性强,简单可行。HPLC法测定丹参素钠在0.29~1.45μg线性关系良好(r=0.9996),平均加样回收率为101.49%,RSD为3.42%;丹酚酸B在0.32~8.00μg线性关系良好(r=0.9999),平均加样回收率为102.17%,RSD为2.17%。10批次丹参药渣水煎液部位相似度均大于0.950,质量较为稳定,没有明显的差异。结论:建立的定性、定量及指纹图谱方法简单,重复性好,结果准确可靠,可用于丹参药渣水煎液部位的质量控制。
Objective: To establish a method for quality control of Salvia miltiorrhiza dregs decoction for protecting endothelial progenitor cells with high glucose. Methods: The TLC and Danshensu sodium and salvianolic acid B were identified qualitatively. The content of Danshensu sodium and Salvianolic acid B in Danshen dregs decoction was determined by high performance liquid chromatography (HPLC). Ten batches of Danshen dregs decoction prepared at different times were determined, and the standard fingerprints of Danshen dregs decoction were established, and the similarity of batches was calculated. Results: TLC is specific and easy to use. The linearity of sodium danshensu was 0.29 ~ 1.45μg (r = 0.9996) by HPLC. The average recovery was 101.49% with RSD of 3.42%. The linear relationship of salvianolic acid B was 0.32 ~ 8.00μg (r = 0.9999 ), The average recovery rate was 102.17%, RSD was 2.17%. 10 batches of Danshen dregs decoction part of the similarity are greater than 0.950, the quality is relatively stable, no significant differences. Conclusion: The established qualitative, quantitative and fingerprinting methods are simple and reproducible, the results are accurate and reliable, which can be used for the quality control of Danshen dregs decoction.