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目的:观察鱼腐败气体中毒后大鼠脑组织神经元特异性烯醇化酶(NSE)、环氧合酶(COX-2)表达的变化,探讨中毒的分子机制。方法:健康SD大鼠30只,分别吸入不同浓度鱼腐败气体,用免疫组化法测定脑组织NSE、COX-2的表达。结果:对照组脑组织可见极少量NSE阳性细胞,但无COX-2阳性表达。中毒组吸入10min后阳性细胞数开始升高,且随着吸入浓度的增加而增多,各组比较存在显著差别(P<0.01)。结论:急性鱼腐败气体中毒对脑组织的损害可能是通过影响NSE、COX-2的表达而实现的。
Objective: To observe the changes of neuron-specific enolase (NSE) and cyclooxygenase (COX-2) in brain tissue of fish poisoned by corrupt gas, and to explore the molecular mechanism of poisoning. Methods: Thirty healthy SD rats were inhaled with different concentrations of fish spoilage gas, and the expression of NSE and COX-2 in brain tissues were determined by immunohistochemistry. Results: There were very few NSE positive cells in the control group but no positive expression of COX-2. The number of positive cells in the poisoning group began to increase 10 min after inhalation, and increased with inhalation concentration, there was significant difference between the groups (P <0.01). Conclusion: The damage to brain tissue caused by gas poisoning by acute fish decay may be caused by affecting the expression of NSE and COX-2.