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目的检测乳头状甲状腺癌(PTC)组织和良性甲状腺肿瘤组织中MGMT基因启动子区甲基化状态,分析MGMT基因甲基化与PTC预后的关联,旨在为探索PTC预后提供依据。方法采用甲基化特异PCR法检测60例PTC及60例良性甲状腺肿瘤组织的MGMT基因启动子区甲基化状态,比较两组的不同病理参数间MGMT基因甲基化状态,并分析其与PTC预后的关系。结果病例组MGMT甲基化阳性率(51.7%)明显高于对照组(26.7%);病例组MGMT甲基化阳性率在有/无吸烟、有/无饮酒间差异显著(P<0.05);年龄越大、肿瘤直径越大、有淋巴结转移、临床分期越高、有MGMT基因甲基化是PTC较差预后的独立影响因素(P<0.05)。结论 MGMT基因启动子区甲基化是PTC重要的早期事件,良性甲状腺肿瘤组织中虽然也可发生,但频率远低于PTC;MGMT基因启动子区甲基化对预后具有提示作用。
Objective To detect the methylation status of MGMT gene promoter region in papillary thyroid carcinoma (PTC) tissues and benign thyroid neoplasms, and to analyze the relationship between MGMT gene methylation and prognosis of PTC in order to explore the prognosis of PTC. Methods Methylation-specific PCR was used to detect the methylation status of MGMT gene promoter in 60 cases of PTC and 60 cases of benign thyroid neoplasms. The methylation status of MGMT gene was compared between two groups and analyzed with PTC Prognosis of the relationship. Results The positive rate of methylation of MGMT in case group was significantly higher than that in control group (51.7%). The positive rate of MGMT methylation in case group was significantly higher than that in non - smoking group (P <0.05). The older, the larger the tumor diameter, lymph node metastasis, the higher the clinical stage, MGMT gene methylation is an independent prognostic factor of PTC independent factors (P <0.05). Conclusion MGMT gene promoter methylation is an important early event in PTC. Although it may occur in benign thyroid tumors, the frequency is much lower than that of PTC. The methylation of MGMT gene promoter may be a prognostic factor.