植物细胞具有全能性,任何组织、细胞、器官都能再生植株,但事实上,不同品种、器官之间的分化能力差异很大,外植体的制备和培养又是建立在无菌操作基础上的一门技术,选择适宜的外植体是确保外植体培养成功的一个重要因素,把好材料的灭菌关是提高接种成功率的关键,因此,在前人研究报道的基础上,现将玉米外植体诱导愈伤组织的培养方法和组培中常用的杀菌剂介绍如下。1 培养方法1.1 幼胚取授粉10～15d,幼胚长约1.0～2.5mm的果穗,去掉外面的苞叶和花丝,浸泡在70％的酒精中消毒灭菌,在无菌条件下,取其幼胚接种在诱 Plant cells are pluripotency, any tissue, cells, organs can regenerate plants, but in fact, different varieties, the ability to differentiate between organs vary greatly, explant preparation and culture is based on aseptic operation The selection of suitable explants is an important factor to ensure the success of explant culture. The key to improve the success rate of vaccination is to sterilize good materials. Therefore, based on the previous reports, The method of culturing callus induced by corn explants and the bactericide commonly used in tissue culture are as follows. 1 culture 1.1 embryos pollination pollination 10 ~ 15d, the young embryo about 1.0 ~ 2.5mm ear, remove the outer husk and filament, soaked in 70% alcohol disinfection, under sterile conditions, whichever is Immature embryos inoculated in the lure