论文部分内容阅读
为探讨脂蛋白脂酶 ( L PL)基因变化与肥胖和脂质代谢的关系 ,采用血凝块提取基因组 DNA,聚合酶链反应和限制性片段长度多态性方法 ( PCR- RFL P)检测 Puv 酶切位点 ,检测对象为 12 5例参加体检的“健康”成年人。结果发现 :1三种基因型及 P+和 P-等位基因在男女性之间无显著差别 ,P+等位基因的频率为 6 3.6 % ,P-等位基因为 36 .4% ,基因型以 P+P+纯合子为最多见。 2 P- P-基因型组的腰臀围比值及血清 Apo B水平较低 ,Apo A/ Apo B比值较高 ,与非 P- P-基因型组相比有显著的统计学差异。提示我国汉族正常人群中存在 L PL Pvu 酶切位点多态性 ,该位点的不同基因型对脂质代谢有影响 ,且可能与体内中心型脂肪分布有关
To investigate the relationship between lipoprotein lipase (LPL) gene changes and obesity and lipid metabolism, genomic DNA extracted by clotting, polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) were used to detect Puv Restriction sites were detected in 125 healthy “adults” who participated in the physical examination. The results showed that there was no significant difference between the three genotypes and the P + and P-allele in males and females. The frequency of P + allele was 6.36% and the P-allele was 36.4% P + P + homozygotes for the most common. The ratio of waist to hip and serum Apo B were lower in the P-P-genotype group than in the non-P-P genotype group, with a higher Apo A / Apo B ratio. This suggests that L PL Pvu restriction site polymorphism exists in Chinese Han normal population, and different genotypes of this locus may affect lipid metabolism and may be related to the distribution of body fat in the body