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ibeB 是一个致脑膜炎大肠杆菌 K1 株中参与大肠杆菌侵袭人脑微血管内皮细胞的基因. 以 ibeB基因转染鼠胚胎干细胞, 结果表明, 大肠杆菌脑微血管内皮细胞侵袭蛋白IbeB不但具有“侵袭”功能, 而且可诱导真核细胞向神经样细胞分化, 经 ibeB 基因稳定转染的鼠胚胎干细胞能特异性表达神经前体细胞标志分子——nestin. GFP 标记的活细胞观察显示外源性 IbeB 蛋白定位于细胞核. 上述结果提示 ibeB基因可能在调控 nestin 表达过程中发挥功能, 这为研究 nestin 基因的表达调控提供了资料.
ibeB is a gene involved in Escherichia coli invasion of human brain microvascular endothelial cells in meningitis-causing K1 strain.IbeB gene was transfected into mouse embryonic stem cells, the results show that the invasion of E. coli brain microvascular endothelial cells IbeB not only “invasion” function , But also induces the differentiation of eukaryotic cells into neuron-like cells. Mouse embryonic stem cells stably transfected with ibeB gene can specifically express the neural precursor cell marker molecule --nestin. The observation of GFP-labeled viable cells shows the localization of exogenous IbeB protein In the nucleus.The above results suggest that the ibeB gene may play a role in the regulation of nestin expression, which provides information for the study of nestin gene expression regulation.