论文部分内容阅读
目的 探讨含CpG的寡脱氧核苷酸(ODN)单独及与5- 氟尿嘧啶(5 -FU)联用对小鼠移植性肝癌的治疗作用。方法 Balb/c小鼠皮下接种肝癌H22 细胞建立荷瘤鼠模型,肿瘤周围皮下分别注射生理盐水(对照组)、无CpG ODN、5 -FU、CpG ODN和CpG ODN+5 FU后,定期测量肿瘤大小。用酶联免疫吸附实验检测鼠血清白细胞介素(IL)- 12和干扰素(IFN) -γ含量;用乳酸脱氢酶释放法检测鼠脾脏自然杀伤(NK)细胞活性。结果 与对照组肿瘤体积[(6710±910) mm3 ]比较, CpG ODN、CpGODN+5- FU及单用5 -FU治疗后,肿瘤体积分别为(3579±481) mm3、(1998±474) mm3 及(2124±434) mm3(P值均<0.01)。对照组鼠血清IL -12 和IFN -γ的含量分别为(238±45) pg/ml和(57±8)pg/ml,与CpG ODN组[IL 12:(464±24) pg/ml;IFN -γ:(134±26) pg/ml]及CpG ODN+5 -FU组[IL -12:(336±29) pg/ml;IFN -γ:(111±5) pg/ml]比较,差异有统计学意义(P值均<0.05)。CpG ODN+5- FU组小鼠血清IL- 12及IFN -γ水平显著高于5- FU组[IL 12:(167±53) pg/ml;IFN -γ:(53±17) pg/ml;P<0.05]。与对照组NK细胞的杀伤活性[(19.2±1.0)%]相比,CpG ODN组[(44.0±1.4)%]及CpG ODN+5 FU组[(30.7±1.3)%]显著增强(P值均<0.05),且CpG ODN+5 FU组显著高于5- FU治疗组[(12.0
Objective To investigate the therapeutic effect of CpG-containing oligodeoxynucleotides (ODN) alone and in combination with 5-fluorouracil (5 -FU) on transplanted liver cancer in mice. Methods Balb / c mice were inoculated subcutaneously with H22 cells to establish a tumor-bearing mouse model. The tumors were injected subcutaneously with normal saline (control group), without CpG ODN, 5-FU, CpG ODN and CpG ODN + 5 FU, size. Serum interleukin (IL) 12 and interferon (IFN) -γ were detected by enzyme-linked immunosorbent assay (ELISA). Natural killer (NK) cell activity was detected by lactate dehydrogenase release assay. Results Compared with the tumor volume in the control group [(6710 ± 910) mm3], the tumor volumes were (3579 ± 481) mm3 and (1998 ± 474) mm3 respectively after treatment with CpG ODN, CpG ODN + 5- And (2124 ± 434) mm3 (P <0.01). The levels of serum IL-12 and IFN-γ in the control group were (238 ± 45) pg / ml and (57 ± 8) pg / ml respectively, compared with those in the CpG ODN group [IL 12: (464 ± 24) pg / IFN-γ: (134 ± 26) pg / ml] and CpG ODN + 5 -FU group compared to IFN-γ: (111 ± 5) pg / ml] The difference was statistically significant (P <0.05). The serum levels of IL-12 and IFN-γ in CpG ODN + 5- FU group were significantly higher than those in 5-FU group [IL 12: (167 ± 53) pg / ml; ; P <0.05]. CpG ODN group [(44.0 ± 1.4)%] and CpG ODN + 5 FU group [(30.7 ± 1.3)%] increased significantly compared with that of control group [(19.2 ± 1.0)%] All <0.05), and CpG ODN + 5 FU group was significantly higher than 5 FU treatment group [(12.0