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目的 了解新型肝炎病毒输血传播病毒 (transfusiontransmittedvirus,TTV)在原发性肝癌 (PLC)患者中的感染状况。方法 在TTV长的ORF保守区设计引物 ,建立巢式聚合酶链反应 (PCR)技术 ,检测了 15 1例原发性肝癌患者血清中TTVDNA。结果 15 1例原发性肝癌患者中有 2 8例TTVDNA阳性 ,阳性率为 18 5 % ;而同时检测 12 5例正常人血清只有 3例TTV阳性 ,阳性率为 2 4 %。结论 TTV在原发性肝癌患者中存在 ,TTVPCR诊断技术对慢性肝炎向肝癌发展具有重要意义
Objective To understand the infection status of new transfusiontransmitted virus (TTV) in patients with primary liver cancer (PLC). METHODS: Primers were designed in TTV long ORF conserved regions to establish nested polymerase chain reaction (PCR) technology. TTV DNA was detected in the serum of 151 patients with primary liver cancer. Results Of the 15 patients with primary liver cancer, 28 were positive for TTV DNA, and the positive rate was 185%. At the same time, 12 patients with normal serum had only 3 cases of TTV positive, and the positive rate was 24%. Conclusion TTV exists in patients with primary liver cancer. TTV PCR diagnosis technique has important significance for the development of chronic hepatitis to hepatocellular carcinoma.