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该文考察了欧前胡素的体外经皮渗透性及影响因素。选择SD大鼠腹皮作为渗透模型,采用改良Franz扩散池法进行体外经皮渗透实验,HPLC测定欧前胡素的含量,考察不同接收液、不同皮肤处理方法及扩散液浓度对欧前胡素稳态渗透速率的影响。结果表明,20%乙醇,0.5%聚氧乙烯脱水山梨醇单油酸酯(吐温-80)均能显著增加欧前胡素的稳态渗透速率;皮肤经20%乙醇、20%乙醇-1%吐温-80处理后,欧前胡素的透皮速率均有所增加,而只经1%吐温-80处理后,欧前胡素的透皮速率反而减小;欧前胡素的稳态渗透速率与扩散液的浓度相关,在低浓度时稳态渗透速率随浓度的增大而增大,当达到一定浓度后,稳态渗透速率无明显变化。由此可见乙醇可以改变皮肤角质层的结构,使药物的稳态渗透速率增加;吐温-80对稳态渗透速率的影响与皮肤结构的改变无关。欧前胡素的经皮渗透受浓度影响,是多种途径扩散的综合结果。
This paper examines the in vitro permeability of imperatorin and its influencing factors. SD rat abdominal skin was chosen as the osmotic model, and the modified Franz diffusion cell method was used for in vitro transdermal permeation test. The content of imperatorin was determined by HPLC. The effects of different receiving solutions, different skin treatment methods and diffusion solution on the stability of imperatorin Infiltration rate effects. The results showed that 20% ethanol and 0.5% polyoxyethylene sorbitan monooleate (Tween-80) could significantly increase the steady infiltration rate of imperatorin. The skin was infiltrated with 20% ethanol, 20% ethanol- Transdermal rate of imperatorin increased after treatment with% Tween-80, whereas the transdermal rate of imperatorin decreased only after treatment with 1% Tween-80; The steady-state permeation rate is related to the concentration of the diffusion solution. At low concentrations, the steady-state permeation rate increases with increasing concentration. After reaching a certain concentration, the steady-state permeation rate does not change significantly. Thus, ethanol can change the structure of the stratum corneum and increase the steady-state permeation rate of the drug. The effect of Tween-80 on the steady-state permeation rate has nothing to do with the change of skin structure. Percutaneous penetration of imperatorin is affected by the concentration and is a composite result of multiple pathways.