论文部分内容阅读
实验证明中草药威灵仙(又名棉团铁线莲(Clematis Mandchurica Rupr)有明显的抗缺氧作用,为了进一步研究其对缺血性心脏病的药理作用,进行了本实验。材料和方法一、药物:威灵仙是在黑龙江省玉泉公社自采的,制成生药1克/毫升的水提酒沉剂备用。垂体后叶素是天津生物化学制药厂制,批号790303,每毫升内含5单位,用蒸馏水稀释成1毫升含0.5单位。二、实验方法:用健康成年雌雄大白鼠20只,按体重分成给药组与对照组,每组10只,实验前将大白鼠用25%乌拉坦0.4ml/100克腹腔注射麻醉,麻醉后将大白鼠背位固定,用国产(上海)XDH—2型心电图机记录Ⅱ导心电图(纸速50mm/秒、电压 lmv=20mm),然后腹腔注射威灵仙水提酒沉剂0.8克/公斤体重,30分钟后,由舌静脉快速注入垂体后叶素0.5单位/公斤体重,记录10秒、20秒、30
Experiments have shown that Clematis Mandchurica Rupr, a Chinese herbal medicine (Clematis Mandchurica Rupr), has obvious anti-hypoxic effect. In order to further study its pharmacological effects on ischemic heart disease, this experiment was performed. Materials and Methods 1 Pharmaceuticals: Clematis is extracted from Yuquan commune in Heilongjiang Province and made into a 1 g/ml water extractant. The pituitrin is manufactured by Tianjin Biochemical Pharmaceutical Factory, batch No. 790303. 5 units, diluted with distilled water to 1 ml containing 0.5 units.II.Experimental method: Using healthy adult male and female rats, divided into administration group and control group according to body weight, 10 rats in each group, and 25% rats before the experiment. Ulantan 0.4ml/100g intraperitoneal injection anesthesia, after immobilization, the rats were fixed in the back position, and II electrocardiograms (paper speed 50mm/sec, voltage lmv=20mm) were recorded with a domestic (Shanghai) XDH-2 electrocardiograph and then abdominal cavity. A total of 0.8 g/kg of body weight was injected with Weilingxian Water Lifting Wine. After 30 minutes, pituitrin was injected into the pituitary at a rate of 0.5 units/kg body weight. Recorded for 10 seconds, 20 seconds, and 30 minutes.