改进了一种基于新型的荧光染料——二氧化硅的核壳荧光纳米颗粒的纳米 p H传感器 ,以异硫氰酸荧光素 ( FITC)标记的羊抗人免疫球蛋白 Ig G为核材料 ,采用实验条件简单的油包水的微乳液方法制备荧光纳米颗粒 ,该方法有效地防止了荧光染料在二氧化硅壳层中的泄露 .这种 FITC的核壳荧光纳米颗粒对 p H敏感 ,在 p H值 5 .5～ 7之间呈线性响应 ,且能被单个小鼠巨噬细胞吞噬 ,借此用于单细胞中 p H的实时监测 A novel nano-p H sensor based on a new fluorescent dye-silica core-shell fluorescent nano-particle was developed. FITC-labeled goat anti-human immunoglobulin Ig G as the core material, Fluorescent nanoparticles were prepared by a simple water-in-oil microemulsion method under experimental conditions, which effectively prevented the leakage of fluorescent dyes in the silica shell. The FITC core-shell fluorescent nanoparticles were sensitive to p H The p H value was linearly responsive to a range of 5.5 to 7 and was phagocytosed by a single mouse macrophage, thereby allowing for the real-time monitoring of p H in single cells