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研究了一种利用新型荧光探针———分子信标进行植物病毒检测的方法 ,可以不要求对病毒RNA进行严格的分离和纯化 ,就能快捷地得到检测结果。此方法被用于烟草花叶病毒 (tobaccomosaicvirus,TMV)基因组RNA的检测 ,为植物病毒分子生物学的研究提供了新的手段
A new method of detecting plant virus using a new fluorescent probe, molecular beacon, has been studied. The detection result can be obtained quickly without requiring stringent isolation and purification of the viral RNA. This method was applied to the detection of Tobacco mosaic virus (TMV) genomic RNA, which provided a new method for the study of plant virus molecular biology