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用ＰＥＧ融合法融合甘薯品种高系１４号（６ｘ）及其近缘野生种Ｉｐｏｍｏｅａｔｒｉｌｏｂａ（２ｘ）的原生质体，融合率达２８％。将融合原生质体培养在含有０．０５ｍｇ·Ｌ－１２，４－Ｄ和０．５ｍｇ·Ｌ－１激动素（ＫＴ）的改良ＭＳ培养基中，４～５ｄ后，再生细胞发生首次细胞分裂。培养１２周后，将直径约２．０ｍｍ的小愈伤组织转移到添加０．０５ｍｇ·Ｌ－１２，４－Ｄ的ＭＳ培养基上培养４周，愈伤组织迅速增殖。将其中的３７个愈伤组织转移到添加０．２ｍｇ·Ｌ－１ＩＡＡ和１．０ｍｇ·Ｌ－１ＢＡＰ的ＭＳ培养基上，２个愈伤组织再生植株。将未再生植株的３５个愈伤组织培养在ＭＳ基本培养基上，１７个愈伤组织再生植株。由其中仅存的７个愈伤组织所获得的再生植株的染色体数为４４～５０，在形态学上相似于Ｉ．ｔｒｉｌｏｂａ，但又有一定变异，表明为体细胞杂种植株。
Protoplast fusion of sweet potato variety GaoXuan No.14 (6x) and its related wild species Ipomoeatriloba (2x) was achieved by PEG fusion method, and the fusion rate was 28%. The fusion protoplasts were cultured in modified MS medium containing 0.05 mg · L-12, 4-D and 0.5 mg · L -1 kinetin (KT). After 4 ~ 5 days, the first division of regenerated cells occurred. After 12 weeks of culture, small callus with diameter of about 2.0 mm was transferred to MS medium supplemented with 0.05 mg · L-12,4-D for 4 weeks, and the callus rapidly proliferated. Thirty-seven of them were transferred to MS medium supplemented with 0.2 mg · L-1 IAA and 1.0 mg · L-1BAP, and two callus were regenerated. Thirty-five callus from non-regenerated plants were cultured on MS basal medium and 17 callus plants were regenerated. The regenerated plants obtained from the remaining seven callus had chromosome numbers of 44-50, which are similar in morphology. Triloba, but with some variation, indicates somatic hybrid plants.