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甲型流感病毒和人免疫缺陷病毒的囊膜蛋白在不同分离株间变异很大,能诱导中和抗体产生的疫苗对不断变化的新分离株没有保护作用,而对保守的病毒抗原具有特异性的细胞毒性T淋巴细胞(CTL)则能对不同的病毒株起反应。在活体内这种CTL的产生通常要求有抗原的内源表达,就象发生在病毒感染时的情况一样。作者将编码甲型流感病毒A/PR/8/34(H1N1)株的核蛋白(NP)的cDNA插入细菌质粒DNA井置于劳斯氏肉瘤病毒或巨细胞病毒启动子的控制之下。将构建的质粒DNA直接注射进BALB/C小鼠的四头肌,结果导致NP特异的CTL的产生并能在随后提供针对甲型流感病毒异源毒株A/HK/68(H3N2)感染的保护。这两种流感病毒毒株的分离时间间隔34年,其表面抗原分属不同的亚型。作者还分别进行了细胞免疫和体液
The enveloped proteins of influenza A and human immunodeficiency virus are highly variable among different isolates and vaccines that induce neutralizing antibody production have no protective effect on a constantly changing new isolate but are specific for conserved viral antigens Of cytotoxic T lymphocytes (CTL) are able to respond to different virus strains. The production of such CTLs in vivo usually requires the endogenous expression of the antigen, as occurs at the time of viral infection. The authors inserted the cDNA encoding the nucleoprotein (NP) of influenza A virus A / PR / 8/34 (H1N1) strain into bacterial plasmid DNA wells under the control of the Rous sarcoma virus or cytomegalovirus promoter. The direct injection of the constructed plasmid DNA into the quadriceps of BALB / C mice resulted in the production of NP-specific CTLs and was subsequently able to provide an additional response to the challenge of the A / HK / 68 (H3N2) infection of influenza A virus heterologous strain A / HK / 68 protection. The two influenza virus strains isolated 34-year intervals, the surface antigens belong to different subtypes. The authors also conducted cellular immunity and body fluid respectively