建立了荧光分光光度法测定红丝线提取物中紫蓝素含量的方法。在pH值为8.0的条件下,荧光激发波长λex=590nm,发射波长λem=625nm处测定样品中紫蓝素的含量。回归方程为y=515.81x+59.496,相关性系数r=0.9996,精密度(RSD)为0.46%(n=7),加标回收率为95.0%—99.4%。该方法用于测定红丝线提取物中紫蓝素的含量结果准确。 A method was established for the determination of the content of purplish blue in the red silk extract by fluorescence spectrophotometry. Under the condition of pH 8.0, the fluorescence excitation wavelength λex = 590nm, the emission wavelength λem = 625nm at the determination of the content of blue dye in the sample. The regression equation was y = 515.81x + 59.496, the correlation coefficient r = 0.9996, the precision (RSD) was 0.46% (n = 7), and the recoveries were 95.0% -99.4%. This method is accurate for the determination of the content of blue-violet in the red thread extract.