建立高效液相色谱法测定和厚朴酚白蛋白纳米粒中和厚朴酚的含量。采用Lichrospher C18(4.6mm×150mm,5μm)色谱柱,流动相为甲醇-水(80∶20,V/V),流速为1.0mL/min,检测波长为294nm,柱温为30℃。在此色谱条件下,和厚朴酚的色谱峰面积与质量浓度在0.048—12.5μg/mL范围内线性关系良好(r=0.9999),平均回收率为101.2%,RSD为0.5%。本法准确可靠、简便易行,可用于和厚朴酚白蛋白纳米粒中和厚朴酚的含量测定。 To establish a method for the determination of honokiol in honokiolbumin nanoparticles by high performance liquid chromatography. The mobile phase was methanol-water (80:20, V / V) at a flow rate of 1.0 mL / min using a Lichrospher C18 column (4.6 mm × 150 mm, 5 μm) with a detection wavelength of 294 nm and a column temperature of 30 ° C. Under the chromatographic conditions, the peak area of ​​honokiol had a good linear relationship (r = 0.9999) with the mass concentration range of 0.048-12.5μg / mL, with an average recovery of 101.2% and a RSD of 0.5%. This method is accurate and reliable, simple and easy, and can be used for the determination of honokiol in honokiolipoprotein nanoparticles.