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目的探讨瑞安市5岁以下婴幼儿病毒性腹泻病原分布以及主要病原流行病学特点。方法采集瑞安市3家医院儿科门诊2012—2013年5岁以下急性腹泻患儿粪便标本,采用聚合酶链式扩增(PCR)及Taq Man荧光探针技术同时进行引起病毒性腹泻的5种主要病毒检测。结果 236份标本中有120份标本检出阳性,检出率为50.85%,其中轮状病毒28.39%、诺如病毒19.49%、札如病毒2.97%、星状病毒2.54%和肠道腺病毒1.69%。1岁~组中,病毒检出率高达62.82%。A组轮状病毒检出率为最高达28.39%,轮状病毒在1月份检出率最高达38.10%,诺如病毒7月份检出率最高达27.27%。结论瑞安市5岁以下婴幼儿病毒性腹泻病原复杂,轮状病毒是最主要病原,其次为诺如病毒。
Objective To investigate the distribution of pathogenic bacteria and the epidemiological characteristics of infant virus diarrhea in infants under 5 years old in Rui’an. Methods Stool samples from children with acute diarrhea under 5 years of age from 2012 to 2013 in pediatric outpatient departments of 3 hospitals in Ruian City were collected. The five main types of virus diarrhea caused by viral diarrhea were detected by polymerase chain reaction (PCR) and Taq Man fluorescent probe Virus detection. Results Of the 236 samples, 120 samples were positive, with a detection rate of 50.85%, including rotavirus 28.39%, Norovirus 19.49%, Sapporo virus 2.97%, Astrovirus 2.54% and Enteric adenovirus 1.69 %. 1 year old ~ group, the virus detection rate as high as 62.82%. The detection rate of rotavirus in group A was up to 28.39%, that in rotavirus was 38.10% in January, and that in Norovirus was 27.27% in July. Conclusions The pathogens of viral diarrhea in infants under 5 years old in Ruian City are complex. Rotavirus is the most important pathogen, followed by norovirus.