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目的：了解干扰素－α（ＩＦＮ－α）逆转白血病细胞多药耐药性（ＭＤＲ）的作用和可能机制。方法：采用链亲和素－胶体金原位杂交（ＳＡＧ－ＩＳＨ）检测了ＩＦＮ－α孵育前后的５２例白血病患者骨髓细胞的多药耐药基因（ＭＤＲ１）表达，用荧光分光光度法测定了ＩＦＮ－α孵育前后的５２例骨髓细胞内柔红霉素（ＤＮＲ）浓度。结果：全部５２例患者中２４例（４６．２％）ＭＤＲ１阳性，初治组与复发难治组相比相差显著。ＭＤＲ１阳性者骨髓细胞内柔红霉素浓度与阴性者相差显著。ＩＦＮ－α孵育后的ＭＤＲ１阳性率与孵育前相差不显著，但经ＩＦＮ－α孵育前后的ＭＤＲ１阳性者的骨髓细胞内ＤＮＲ浓度相差显著，阴性者亦然。结论：ＩＦＮ－α能增加白血病细胞内ＤＮＲ浓度，其逆转ＭＤＲ环节不在ＭＤＲ１基因水平上，而在其他耐药途径上
Objective: To investigate the role and possible mechanism of interferon-α (IFN-α) in reversing multidrug resistance (MDR) of leukemia cells. METHODS: The expression of multidrug resistance gene (MDR1) in bone marrow cells of 52 leukemia patients before and after incubation with IFN-α was detected by streptavidin-colloidal gold in situ hybridization (SAG-ISH). The expression of MDR1 was determined by fluorescence spectrophotometry. The concentration of daunorubicin (DNR) in 52 bone marrow cells before and after IFN-α incubation. RESULTS: Of the 52 patients, 24 (46.2%) were MDR1 positive. The difference between the untreated group and the relapsed and refractory group was significant. The concentration of daunorubicin in bone marrow cells of MDR1-positive patients was significantly different from that of negative ones. The positive rate of MDR1 after incubation with IFN-α was not significantly different from that before incubation, but there was a significant difference in DNR concentration between bone marrow cells of MDR1-positive patients before and after incubation with IFN-α, and negative was also found. Conclusion: IFN-α can increase the concentration of DNR in leukemia cells, and its reversal of MDR is not at the level of MDR1 gene, but in other drug resistance pathways.