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目的探讨NDRG2基因在人原发性肝细胞肝癌及正常肝组织中的表达及其临床意义。方法收集原发性肝细胞肝癌及正常肝组织标本,采用免疫组化ABC法、Western blot法及Real-time PCR法检测原发性肝细胞肝癌及正常肝组织标本中NDRG2蛋白和mRNA的表达情况,并结合图像分析,比较两者表达的差异。结果 NDRG2蛋白在肝癌组织及正常肝组织中的表达阳性率分别为16.67%(5/30)和100%(30/30),2组差异有统计学意义(P<0.05),且阳性表达呈现在细胞质中,细胞核中未见有表达。图像分析结果显示,NDRG2蛋白在肝癌组织中表达的相对含量为0.029 0±0.005 9,在正常肝组织中为0.109 2±0.002 8,前者低于后者,其差异具有统计学意义(P<0.05)。Western blot法检测结果与免疫组化染色结果相一致,在肝癌组织及正常肝组织中均检测到NDRG2蛋白,肝癌组织中NDRG2蛋白相对表达量为1.13±0.15,低于正常肝组织的1.57±0.18(P<0.05)。Real-time PCR分析结果显示肝癌组织中NDRG2 mRNA表达量为0.89±0.15,低于正常肝组织的1.48±0.17(P<0.05),但肝癌Edmondson-Steiner分级的各级肝癌组织之间NDRG2 mRNA表达量差异均无统计学意义(P>0.05)。结论 NDRG2基因在原发性肝细胞肝癌及正常肝组织中可能存在差异性表达,提示该基因可能参与了肝细胞肝癌的发生和发展,但其具体作用及调控机理有待进一步研究。
Objective To investigate the expression of NDRG2 gene in human primary hepatocellular carcinoma (HCC) and normal liver tissue and its clinical significance. Methods Primary hepatocellular carcinoma and normal liver tissue samples were collected. Immunohistochemical ABC method, Western blot and Real-time PCR were used to detect the expression of NDRG2 protein and mRNA in primary hepatocellular carcinoma and normal liver tissues , And combined with image analysis to compare the differences between the two expression. Results The positive rates of NDRG2 protein expression in HCC tissue and normal liver tissue were 16.67% (5/30) and 100% (30/30), respectively. The positive rates of NDRG2 protein in the two groups were statistically significant (P <0.05) In the cytoplasm, there is no expression in the nucleus. The results of image analysis showed that the relative content of NDRG2 protein was 0.029 0 ± 0.005 9 in normal liver tissue and 0.109 2 ± 0.002 8 in normal liver tissue, the former was lower than the latter, the difference was statistically significant (P <0.05 ). NDRG2 protein was detected in both HCC tissues and normal liver tissues. The relative expression of NDRG2 protein in HCC tissues was 1.13 ± 0.15, which was lower than that of normal liver tissues (1.57 ± 0.18) (P <0.05). Real-time PCR analysis showed that the expression of NDRG2 mRNA in HCC tissues was 0.89 ± 0.15, which was lower than that in normal liver tissues (1.48 ± 0.17, P <0.05). However, NDRG2 mRNA expression was significantly different between HCC Edamson-Steiner grade liver cancer tissues There was no significant difference between the two groups (P> 0.05). Conclusion The NDRG2 gene may be differentially expressed in primary hepatocellular carcinoma and normal liver tissue, suggesting that the gene may be involved in the occurrence and development of hepatocellular carcinoma. However, the specific role and regulation of NDRG2 remains to be further studied.