论文部分内容阅读
目的探讨模拟400 m氦氧饱和潜水对大鼠肺氧化与抗氧化系统的影响。方法将SD大鼠在4.1 MPa(绝对压力)氦氧高气压环境暴露24 h,以常压空气环境作为对照。检测肺组织病理学,测定肺组织丙二醛(MDA)、8-羟基脱氧鸟苷(8-OHdG)、还原型谷胱甘肽(GSH)含量及超氧化物歧化酶(SOD)与谷胱甘肽过氧化物酶(GSH-Px)活力。结果氦氧暴露大鼠肺组织病理学检查未见异常;肺组织匀浆中MDA、8-OHdG含量与SOD活力无明显变化,GSH-Px活力及GSH含量显著降低(P<0.05)。结论 4.1 MPa氦氧暴露24 h使大鼠肺处于氧化应激状态,抗氧化能力降低,但未造成明显的氧化损伤。
Objective To investigate the effects of simulated 400-m He-O-2 saturation diving on lung oxidation and anti-oxidative system in rats. Methods Sprague-Dawley rats were exposed to 4.1 MPa (absolute pressure) Helium-Oxygen pressure for 24 h. Atmospheric air pressure was used as a control. The lung histopathology was examined to determine the contents of malondialdehyde (MDA), 8-OHdG, GSH and SOD in lung tissue and the content of glutathione Glycopeptide peroxidase (GSH-Px) activity. Results There was no abnormality in the pathological examination of lungs exposed to helium-exposed rats. The contents of MDA and 8-OHdG in lung homogenate did not change significantly with the activity of GSH-Px and the content of GSH in lungs significantly decreased (P <0.05). Conclusions Exposure to helium and oxygen for 24 h at 4.1 MPa exposes rats to oxidative stress in the lungs with reduced antioxidant capacity but no significant oxidative damage.