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目的比较不同剂量多杀菌素Natular G30在生活污水与小型净水中对致倦库蚊海口种群的控制效果,为其在蚊虫控制中的应用提供依据。方法用海南省海口市生活污水与小型净水中的致倦库蚊幼虫和水,在外环境以减退率为指标,分别测定Natular G30 10、20、40和80 mg/m2对蚊虫的控制效果。试验中,两次测量试验水体的水质pH值、温度、总固体含量、盐度、溶解氧含量。结果生活污水的pH值、溶解氧含量分别为(9.62±0.43)、(12.79±3.01)mg/L,小型净水分别为(8.91±0.39)、(7.77±3.46)mg/L,二者差异有统计学意义(P<0.05)。在生活污水中,多杀菌素有效浓度为10和20 mg/m2时,蚊虫控制持效时间为12 d,40和80 mg/m2处理的则>30 d;而在小型净水中,所有4个浓度均有超过30 d的持效期。两种水体所有剂量在第1天对1、2龄期幼虫的减退率即接近100%,3、4龄期幼虫在第3~7天出现100%减退,而蛹在第1天的减退率基本为负值。结论多杀菌素Natular G30对致倦库蚊幼虫的控制效果具有高效、持久的特点;且对1、2龄幼虫的毒性高于对3、4龄幼虫的毒性,对蛹无明显的毒杀作用。因其在小型净水中的效果优于生活污水,使用时应根据水质调整剂量。
Objective To compare the control effects of different doses of spinosad on the Haikou Population of Culex pipiens quinquefasciatus in domestic sewage and small water purification, and provide basis for its application in mosquito control. Methods The control effects of 10, 20, 40 and 80 mg / m2 Natratie G30 on mosquitoes were determined with the reduction rate of Culex pipiens pallens larvae and water in domestic sewage and small clean water in Haikou, Hainan Province. In the experiment, the water quality pH value, temperature, total solid content, salinity, dissolved oxygen content of the test water body were measured twice. Results The pH value and dissolved oxygen content of domestic sewage were (9.62 ± 0.43) and (12.79 ± 3.01) mg / L, respectively, while those of small clean water were (8.91 ± 0.39) and (7.77 ± 3.46) mg / L respectively There was statistical significance (P <0.05). In domestic sewage, when the effective concentrations of spinosad were 10 and 20 mg / m2, the control effect time of mosquitoes was 12 days and that of 40 and 80 mg / m2 was> 30 days; while in small water purification, all 4 A concentration of more than 30 d of the persistence period. At day 1, the reduction rates of all the larvae on the first and second instar larvae were nearly 100%. The larvae of the third instar larvae showed 100% decrease on the third and seventh days, and the rate of decrease of pupae on the first day The basic negative. Conclusion The control effect of spinosad on the larvae of Culex pipiens quinquefasciatus is highly efficient and durable. The toxicity to the first and second instar larvae is higher than that of the third and fourth instar larvae, and no toxic effect on the pupae . Because of its effect in small water purification is better than domestic sewage, the dosage should be adjusted according to water quality.