论文部分内容阅读
目的建立检测壳聚糖(chitosan,CTS)含量的紫外分光光度法,并进行验证。方法通过对反应体系溶液及标准曲线稀释倍数的优化,建立紫外分光光度法检测CTS含量,并对该方法进行专属性、线性范围、准确度、精密度(重复性、中间精密度)及耐用性验证。结果确定以0.2%乙酸溶液作为反应体系溶液,2倍系列稀释作为标准曲线的稀释倍数。该方法检测0.2%乙酸溶液组CTS的回收率为8.07%,而CTS溶液组的回收率可达101.38%;该方法的线性范围为0~62.5μg/ml;CTS溶液浓度在25.0~75.0μg/ml之间,该方法具有良好的准确度;重复性、中间精密度及耐用性检测结果的变异系数(CV)均<20%。结论建立了CTS含量紫外分光光度检测方法,该方法具有良好的专属性、重复性及中间精密度,且在25.0~62.5μg/ml具有良好的准确度,0~62.5μg/ml具有良好的线性。
Objective To establish a UV spectrophotometric method for the determination of chitosan (CTS) content. Methods The CTS content was determined by UV spectrophotometry with the optimization of the dilution of the reaction system solution and standard curve. The specificity, linearity, accuracy, precision (repeatability, intermediate precision) and durability verification. The results determined to 0.2% acetic acid solution as the reaction system solution, 2-fold dilution series as the standard curve dilution. The method showed that the recovery of CTS was 8.07% in 0.2% acetic acid solution and 101.38% in CTS solution. The linear range of this method was 0 ~ 62.5μg / ml; the concentration of CTS was 25.0 ~ 75.0μg / ml, the method has good accuracy; the coefficient of variation (CV) of repeatability, intermediate precision and durability test results are both <20%. Conclusion The UV spectrophotometric method for the determination of CTS content is established. The method has good specificity, repeatability and intermediate precision. The method has good accuracy at 25.0 ~ 62.5μg / ml and good linearity at 0 ~ 62.5μg / ml .