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目的探讨华支睾吸虫病患者白细胞介素-6(IL-6)、白细胞介素-10(IL-10)、肿瘤坏死因子-α(TNF-α)水平与肝功能的临床关系。方法试验组为50例未经治疗的华支睾吸虫病患者,并参照ChildPugh肝功能分级法,按照评分将其分成Ⅰ、Ⅱ、Ⅲ三组;对照组为20例健康献血员。采集试验组及对照组静脉血,用全自动生化分析仪检测血浆中总胆红素(TBIL)、血清白蛋白(ALB)和谷丙转氨酶(ALT)含量;采用放射免疫法检测血清IL-6、IL-10及TNF-α含量,并进行统计分析。结果华支睾吸虫病患者血清IL-6、IL-10、TNF-α水平明显高于健康人对照组(P<0.01)。随着患者肝功能受累程度的加重,血清IL-6、IL-10及TNF-α含量依次递增,Ⅰ组和Ⅲ组间差异均有统计学意义(P<0.01)。IL-6、TNF-α含量与其TBIL(γ=0.470、P<0.01,γ=0.518、P<0.01)及ALT(γ=0.497、P<0.01,γ=0.285、P<0.05)呈正相关,与ALB呈负相关(γ=-0.620、P<0.01,γ=-0.665、P<0.01)。结论华支睾吸虫病患者IL-6和TNF-α表达增强,共同参与介导了华支睾吸虫病肝损伤过程。
Objective To investigate the clinical relationship between interleukin-6 (IL-6), interleukin-10 (IL-10), tumor necrosis factor-α (TNF- α) and liver function in patients with clonorchiasis. Methods Fifty untreated clonorchiasis patients were enrolled in the study. According to the ChildPugh classification of liver function, the experimental group was divided into three groups Ⅰ, Ⅱ and Ⅲ. The control group consisted of 20 healthy blood donors. Blood samples were collected from the test group and the control group. The levels of total bilirubin (TBIL), serum albumin (ALB) and alanine aminotransferase (ALT) were measured by automatic biochemical analyzer. The levels of IL-6 , IL-10 and TNF-α content, and statistical analysis. Results Serum levels of IL-6, IL-10 and TNF-α in patients with clonorchiasis were significantly higher than those in healthy controls (P <0.01). The levels of IL-6, IL-10 and TNF-α in serum increased gradually with the degree of hepatic involvement. The difference between group Ⅰ and group Ⅲ was statistically significant (P <0.01). The levels of IL-6 and TNF-α were positively correlated with TBIL (γ = 0.470, P <0.01, γ = 0.518, P <0.01) and ALT (γ = 0.497, P <0.01, ALB was negatively correlated (γ = -0.620, P <0.01, γ = -0.665, P <0.01). Conclusion The expression of IL-6 and TNF-α in patients with Clonorchiasis sinensis is enhanced, and they participate in the process of hepatic injury induced by Clonorchis sinensis.