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目的:研究蛇床子素对离体豚鼠心房的作用及与Ca~(2+)的关系.方法:钙拮抗剂Ver对照,电刺激左房收缩,累积浓度法测定给Ost前后左房收缩幅度,给药间隔15 min,计算pA_2或pD_2′;观察Ost对左房收缩正阶梯、静息后增强作用。收缩反应由XWT-204台式平衡记录仪描记.结果:Ost、Ver产生浓度依赖性负性肌力作用,抑制高K~+Iso恢复的左房收缩;非竞争性拮抗Iso、CaCl_2正性肌力作用,相应pD_2′值分别为4.41±0.13和6.53±0.22,4.90±0.15和6.91±0.17;抑制正阶梯,大剂量使之翻转,对静息后增强作用弱.结论:Ost对心房抑制作用与Ver相似,但强度较后者弱,可能抑制胞外钙内流.
Objective: To study the effect of osthole on isolated guinea pig atria and its relationship with Ca 2+ . Methods: Calcium antagonist Ver control, electrical stimulation of left atrial contraction, cumulative concentration method to determine the amplitude of left atrium before and after Ost, Dosing interval 15 min, calculate pA 2 or pD 2 ’; observe the positive effect of Ost on the positive contraction of left atrium contraction and post rest. The contractile response was measured by an XWT-204 table balance recorder. Results: Ost and Ver produced a concentration-dependent negative inotropic effect, inhibited left atrial contraction with high K++Iso recovery; non-competitively antagonized Iso, CaCl 2 positive inotropic The corresponding pD_2′ values were 4.41±0.13 and 6.53±0.22, 4.90±0.15 and 6.91±0.17, respectively; the positive ladder was inhibited and the large dose was reversed, and the enhancement effect after resting was weak. Conclusion: The effect of Ost on atrial inhibition Similar to Ver, but weaker than the latter, may inhibit extracellular calcium influx.