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采用喷雾接种的方法测定了不同浓度的水杨酸和壳聚糖对苹果斑点落叶病菌(Alternaria mali Roberts)的室内抑菌作用、诱导抗病效果及诱导苹果叶片后各种抗病性物质含量的变化。结果表明,不同浓度的水杨酸和壳聚糖对A.mali均无明显的抑制作用,其中100μg/mL的水杨酸和500μg/mL的壳聚糖对A.mali的抑制率较低,仅为5.33%和4.73%,但诱导抗病效果较显著,分别达到70.90%和77.77%。两种药剂诱导叶片后,叶片中POD、PAL活性以及木质素积累量明显高于对照,且随时间的增长,POD和PAL酶活性先升高后降低,在第3天,POD和PAL酶活性均达到峰值;而壳聚糖诱导后PPO活性随时间的增长呈升高的趋势,但水杨酸对PPO酶活性变化的影响较小。
The antibacterial activity of salicylic acid and chitosan on Alternaria mali Roberts was determined by spray inoculation, and the anti-pathogenic effect and the content of various disease-resistant substances after induction of apple leaf were determined Variety. The results showed that different concentrations of salicylic acid and chitosan had no significant inhibitory effect on A.mali, of which salicylic acid at 100μg / mL and chitosan at 500μg / mL had a lower inhibition rate on A.mali, Only 5.33% and 4.73% respectively, but the effect of inducing disease resistance was more remarkable, reaching 70.90% and 77.77% respectively. The activities of POD and PAL and the accumulation of lignin in leaves were significantly higher than those in the control after two kinds of chemical agents were induced, and the activity of POD and PAL increased at first and then decreased with the increase of time. On the third day, the activity of POD and PAL All reached its peak. However, the activity of PPO increased with the increase of time after induced by chitosan, but salicylic acid had little effect on the change of PPO activity.