激光3号为扬州生化制药厂生产,生理盐水配制。接种11天的Lewis肺癌小鼠,按40mg/kg体重由尾静脉注射激光3号,给药后12、24、48和72小时处死动物,取肿瘤、皮肤、肌肉及有关脏器分别制成匀浆,用正丁醇提取其中的血卟啉化合物,用日本MPF-4型荧光分光光度计测定含量。测定样品用红外灯烤至恒重。 Laser No. 3 was produced by Yangzhou Biochemical Pharmaceutical Factory and formulated with normal saline. Lewis lung cancer mice that were inoculated for 11 days were injected with laser No. 3 at a dose of 40 mg/kg body weight from the tail vein. Animals were sacrificed at 12, 24, 48, and 72 hours after administration. Tumors, skin, muscles, and related organs were prepared separately. In the slurry, the hematoporphyrin compound was extracted with n-butanol, and the content was measured with a Japan MPF-4 fluorescence spectrophotometer. The sample was roasted with an infrared lamp to constant weight.