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[目的]研究勋章菊叶片再生技术,筛选适合勋章菊叶片再生的最佳培养配方。[方法]以日本引进的勋章菊叶片为材料,置床于添加不同种类和浓度激素的MS培养基中进行愈伤组织和不定芽诱导的正交试验,筛选勋章菊叶片再生的最佳培养基配方。[结果]在MS+TDZ(0.8～1.0mg/L)+NAA(0.05～1.0mg/L)培养基上可形成紧密型绿色的愈伤组织;叶片接种于MS+TDZ(0.5～1.0mg/L)+NAA(0.05～1.0mg/L)培养基上可形成许多不定芽,诱导效率达100%;健壮的不定芽长至2.0～3.0cm长时移至1/2MS+NAA(0.1mg/L)培养基中,其发根状况良好,生根率达100%。[结论]为勋章菊的高频快繁提供了新途径,并为勋章菊的遗传转化及培育新品种奠定基础。
[Objective] The research aimed to study the regeneration technology of medlar chrysanthemum leaves and screen the best culture formula suitable for the regeneration of medlar chrysanthemum leaves. [Method] With the medlar leaves introduced from Japan as material, the orthogonal experiment was used to induce callus and adventitious buds in MS medium supplemented with different kinds and concentrations of hormones, and the optimal culture medium for medlar leaf regeneration . [Result] Tight green callus could be formed on MS + TDZ (0.8-1.0 mg / L) + NAA (0.05-1.0 mg / L) L) + NAA (0.05 ~ 1.0mg / L) medium, inducing efficiency of 100%; robust adventitious buds grow to 2.0 ~ 3.0cm long time to 1 / 2MS + NAA (0.1mg / L) medium, the rooting condition is good, rooting rate of 100%. [Conclusion] The study provided a new way for the high-frequency rapid propagation of medlar, and laid the foundation for the genetic transformation of medaka and cultivating new varieties.